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KMID : 0644319980040010131
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Journal of Korean Oriental Oncology
1998 Volume.4 No. 1 p.131 ~ p.146
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Anti-metastatic Effects of Shiquandabutang on Human Fibrosarcoma cells, HT1080
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윤재호/Yoon, Jae-Ho
최승훈/안규석/Choi, Seung-Hoon/Ahn, Kyoo-Seok
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Abstract
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To examine the effect of Shiquandabutang on the metastasis of cancer, the following experiments were made. Before the main experiments, the cytotoxieity was measured by putting Shiquandabutang sample in HT1080, Then zymography was made to examine the change of gelatinolytic activity. And western blotting was carried out to examine the changes of Fos, Jun, Ets, the transcription factors of MMP-2. MMP-9, and Erk. JNK on signal transduction pathway to AP-1, Third, in vitro invasion assay with tranwells coated by collagen and matrigel was carried out. From the results of the above the following conclusions were obtained.
1. The experimental result about cytotoxicity of Shiquandabutang against HT1080 was as below. The stained cell count after being treated by Shiquandabutang sample 400ug/ml for 24 hours was 0.9% of total cells. and the stained cell count by Shiquandabutang sample 100ug/ml was 1.5% of total cells. Both were near the level ot control groupwhich showed 0.6% stained.
2. The restlt of collagenase assay was as bclow. In shiquandanbutang sample 400ug/ml, MMP-2 was reduced as compared with TPA control group. and the band of MMP-9 induced by TPA disappeared. In Shiquandabutang sample 800ug/ml. both bands of MMP-2 and MMP-9 disappeared.
3. The reasults of western blots for Jun, Fos, Ets, Erk, JNK were as below. In Shiwaandabutang sample 200ug/ml, Ets was reduced, and Jun, Fos were increased.
4. The result of invasion assay was as below. The number of cells which migrated across transwell membrane in
shiquandabutang-lteated group was less than that of +TPA control group.
From the above results, it was concluded that Shiquandabutang might control the appearting and acting of collagenase not by the MMP-2, -9 promoter but by other way.
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KEYWORD
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